6PBP
Pseudopaline Dehydrogenase with (S)-Pseudopaline Soaked 1 hour
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.633, 53.758, 96.802 |
| Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
| Resolution | 39.890 - 1.640 |
| R-factor | 0.1696 |
| Rwork | 0.169 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c4n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.890 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 110273 | 4450 |
| <I/σ(I)> | 12.8 | 2 |
| Completeness [%] | 97.4 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.980 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 290 mM ammonium formate, and 28% PEG 3350 |
