6PBL
Crystal structure of Malate dehydrogenase from Legionella pneumophila Philadelphia 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-25 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.560, 79.790, 152.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.810 | 39.810 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.052 | 0.027 | 0.454 |
Rmeas | 0.057 | 0.030 | 0.554 |
Total number of observations | 323671 | ||
Number of reflections | 60783 | 772 | 4415 |
<I/σ(I)> | 18.13 | 41.04 | 2.2 |
Completeness [%] | 99.9 | 97.2 | 99.8 |
Redundancy | 5.325 | 6.234 | 3.017 |
CC(1/2) | 0.999 | 0.999 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 285 | RigakuReagents JCSG+ screen, D6: 20% (w/V) PEG 8000, 200mM MgCl2, 100mM Tris base / HCl pH 8.5: LepnA.01212.a.B1.PS38424 at 20mg/ml + 4mM NAD + 4mM sodium malonate pH 7.0. Cryo: 20% EG: tray 300234: puck svh3-10. |