6PB3

Structure of Rhizobiales Trip13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 6
Unit cell lengths	100.436, 100.436, 48.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	86.980 - 2.048
R-factor	0.2106
Rwork	0.208
R-free	0.25170
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.829
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.980	2.100
High resolution limit [Å]	2.048	2.050
Rmerge	0.058	1.955
Rmeas	0.061	2.057
Rpim	0.019	0.635
Number of reflections	17737	1347
<I/σ(I)>	17.3	0.9
Completeness [%]	99.3	99
Redundancy	10.2	10.2
CC(1/2)	0.999	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM sodium citrate pH 6.0, 1.6 M NH2SO4, and 0.2 M sodium/potassium tartrate