6PAZ

OXIDIZED MUTANT P80I PSEUDOAZURIN FROM A. FAECALIS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1994-04
Detector	SIEMENS
Spacegroup name	P 65
Unit cell lengths	50.790, 50.790, 98.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.260 - 1.910
R-factor	0.175 *
RMSD bond length	0.005
RMSD bond angle	0.016
Data reduction software	XENGEN
Data scaling software	XENGEN
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.260	2.000
High resolution limit [Å]	1.910	1.910
Rmerge	0.038	0.110
Number of reflections	10151
<I/σ(I)>	20.4	3.4
Completeness [%]	97.0 *	80 *
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.8 *		50MM SODIUM PHOSPHATE WITH 75%, pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	phosphate	20 (mM)
3	1	1	ammonium sulfate	42 (%sat)