6PAL

Bacteroides uniformis endo-laminarinase BuGH158 from the beta(1,3)-glucan utilization locus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	65.675, 106.030, 126.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.726 - 1.818
Rwork	0.169
R-free	0.20580
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.561
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.726	1.849
High resolution limit [Å]	1.818	1.818
Rmerge	0.113	1.479
Rmeas	0.117	1.560
Rpim	0.032	0.490
Number of reflections	80471	3966
<I/σ(I)>	14.8	1.4
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M bicine pH 9.0 1.6 M ammonium sulfate