6PAJ
Structure of the SrrAB Histidine Kinase DHp-CA domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.979,0.9505 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.212, 58.952, 70.860 |
Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
Resolution | 29.920 - 2.000 |
R-factor | 0.1899 |
Rwork | 0.187 |
R-free | 0.24610 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.455 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.19) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 69.082 | 69.082 | 2.100 |
High resolution limit [Å] | 1.990 | 6.290 | 1.990 |
Rmerge | 0.052 | 0.039 | 0.346 |
Rmeas | 0.061 | 0.045 | 0.400 |
Total number of observations | 132910 | 3742 | 19809 |
Number of reflections | 33780 | 996 | 4923 |
<I/σ(I)> | 19.9 | 47.2 | 3.7 |
Completeness [%] | 99.5 | 88.8 | 99.9 |
Redundancy | 3.9 | 3.8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 20% PEG4000, 15% MPD, 0.1 M sodium citrate pH 5.6 |