6PA1
Killer cell immunoglobulin-like receptor 2DL2 in complex with HLA-C*07:02
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.565, 82.086, 104.887 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 32.321 - 3.010 |
| R-factor | 0.2655 |
| Rwork | 0.263 |
| R-free | 0.30960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.711 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 20697 | 2015 |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 97.0 | 90.5 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium tartrate dibasic dihydrate and 20% w/v PEG 3350 |
