6P94

Human APE1 C65A AP-endonuclease product complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-17
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	43.585, 60.459, 71.924
Unit cell angles	83.69, 79.47, 88.74

Refinement procedure

Resolution	24.579 - 2.090
R-factor	0.1894
Rwork	0.186
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dff
RMSD bond length	0.009
RMSD bond angle	1.091
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.581	2.142
High resolution limit [Å]	2.090	2.090
Rmerge	0.152
Rmeas		0.798
Number of reflections	36463	611
<I/σ(I)>	7.81
Completeness [%]	99.2
Redundancy	3.6
CC(1/2)		0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% PEG20000, 100 mM sodium citrate, pH 5.0, and 200 mM MgCl2