6P8P

Structure of P. aeruginosa ATCC27853 HORMA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 4
Unit cell lengths	97.525, 97.525, 60.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.525 - 1.635
R-factor	0.1746
Rwork	0.173
R-free	0.19840
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.530	1.660
High resolution limit [Å]	1.635	1.635
Rmerge	0.093	1.786
Rmeas	0.101	1.949
Rpim	0.039	0.768
Number of reflections	69749	3430
<I/σ(I)>	15	1.1
Completeness [%]	99.7	95.4
Redundancy	6.6	6.1
CC(1/2)	0.999	0.445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM imidazole pH 8.0, 200 mM CaCl2, and 32% PEG 3350