6P8F

Crystal structure of CDK4 in complex with CyclinD1 and P27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	62.855, 66.628, 184.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.060 - 2.890
R-factor	0.189
Rwork	0.186
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.060	3.070
High resolution limit [Å]	2.890	2.890
Rmerge	0.180	0.990
Rpim	0.056	0.296
Number of reflections	18044	2830
<I/σ(I)>	14.3	2.9
Completeness [%]	99.8
Redundancy	12.9	12.8
CC(1/2)		0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	100mM Tris (7.0) 17% PEG 3350 100 mM CaCl2 10 mM MgCl2