6P82
Structure of P. aeruginosa ATCC27853 CdnD, Apo form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 104.589, 117.387, 147.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.923 - 2.050 |
| R-factor | 0.2142 |
| Rwork | 0.213 |
| R-free | 0.24260 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.825 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.800 | 2.080 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.138 | 2.033 |
| Rmeas | 0.150 | 2.234 |
| Rpim | 0.058 | 0.906 |
| Number of reflections | 113653 | 5059 |
| <I/σ(I)> | 10.5 | 0.7 |
| Completeness [%] | 99.5 | 89.9 |
| Redundancy | 6.6 | 5.3 |
| CC(1/2) | 0.997 | 0.336 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5 and 1.8-2.0 M Ammonium sulfate |
