6P7Y

Crystal Structure of the Cedar henipavirus Attachment G Glycoprotein globular domain in complex with the receptor ephrin-B2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9192
Spacegroup name	P 65
Unit cell lengths	207.021, 207.021, 120.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.973 - 2.844
R-factor	0.2076
Rwork	0.207
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.386
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.850	6.140	2.850
Rmerge	0.112	0.047
Rmeas	0.121	0.051
Rpim	0.053	0.019	0.628
Total number of observations	505212
Number of reflections	69486	7096	6883
<I/σ(I)>	6.7
Completeness [%]	100.0	100	100
Redundancy	7.3	7.2	6.9
CC(1/2)		0.999	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	100 mM Tris, pH 8.0, 18% PEG5000 MME