6P7L
1.8 Angstrom structure of Aln2 from Streptomyces sp. CM020
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.201, 84.932, 104.655 |
| Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
| Resolution | 42.720 - 1.800 |
| R-factor | 0.1652 |
| Rwork | 0.163 |
| R-free | 0.20550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bka 6p77 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.118 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.720 | 1.865 |
| High resolution limit [Å] | 1.800 | 1.801 |
| Number of reflections | 78418 | 7692 |
| <I/σ(I)> | 10.14 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.992 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 22% PEG 3350 0.1 M Bis Tris ph 5.5 3% ethylene glycol |
