6P6E
Structure of Mouse Importin alpha - PAC3 NLS peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.425 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.490, 90.495, 99.716 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.669 - 1.990 |
R-factor | 0.1755 |
Rwork | 0.175 |
R-free | 0.19660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ukw |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.669 | 2.065 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.118 | |
Rmeas | 0.122 | |
Rpim | 0.034 | 0.720 |
Number of reflections | 49103 | 93402 |
<I/σ(I)> | 18.29 | |
Completeness [%] | 99.8 | 98.78 |
Redundancy | 13 | |
CC(1/2) | 1.000 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | Sodium Citrate, DTT, ph 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |