6P6A

Structure of Mouse Importin alpha - NIT2 NLS peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.425
Spacegroup name	P 21 21 21
Unit cell lengths	78.678, 90.180, 99.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.568 - 2.151
R-factor	0.1672
Rwork	0.166
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u5p
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.570	2.228
High resolution limit [Å]	2.151	2.151
Rmerge	0.116	1.056
Rmeas	0.121
Rpim	0.034	0.321
Number of reflections	38952	3788
<I/σ(I)>	2.28	0.819
Completeness [%]	99.8	99.11
Redundancy	12.7	11.6
CC(1/2)	0.999	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K