6P4Z

Structure of gadolinium-caged cobalt (III) insulin hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	81.400, 81.400, 33.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.498 - 1.800
R-factor	0.1839
Rwork	0.181
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.500	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	7216	1153
<I/σ(I)>	13.5
Completeness [%]	92.9
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% (w/v) PEG 12,000 and 0.1 M HEPES pH 7.0