6P4O

Complex of XPB helicase with Bax1 endonuclease from Sulfurisphaera tokodaii at 3.15 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	217.723, 125.851, 148.970
Unit cell angles	90.00, 101.52, 90.00

Refinement procedure

Resolution	29.930 - 3.150
R-factor	0.2657
Rwork	0.265
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tnu
RMSD bond length	0.007
RMSD bond angle	1.328
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	145.969	29.914	3.320
High resolution limit [Å]	3.150	9.960	3.150
Rmerge		0.070	0.705
Rmeas	0.161	0.078	0.814
Rpim	0.072	0.033	0.397
Total number of observations	258254	10834	29065
Number of reflections	57984	2105	7861
<I/σ(I)>	6.6	17.1	1.7
Completeness [%]	85.0	94.4	79.5
Redundancy	4.5	5.1	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1 M Tris-Cl, pH 8.0, 50 mM Sodium Carbonate, 32% PEG 400