6P3Z
Crystal Structure of Full Length APOBEC3G E/Q (pH 5.2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.788, 153.513, 67.800 |
Unit cell angles | 90.00, 114.14, 90.00 |
Refinement procedure
Resolution | 48.171 - 2.844 |
R-factor | 0.2071 |
Rwork | 0.204 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 27992 | 2278 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 94.3 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 291 | 50 mM MES, pH 5.2 1.7 M lithium sulfate 10 mM MgCl2 |