6P3Y

Crystal Structure of Full Length APOBEC3G E/Q (pH 7.4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	67.695, 153.849, 67.728
Unit cell angles	90.00, 116.37, 90.00

Refinement procedure

Resolution	39.169 - 2.570
R-factor	0.1978
Rwork	0.196
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.670
High resolution limit [Å]	2.580	2.580
Number of reflections	38586	3355
<I/σ(I)>	15.4
Completeness [%]	98.1
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	50 mM Tris, pH 7.4 1.9 M ammonium sulfate 25 mM MgCl2