6P3B
Crystal structure of the anti-HIV antibody DH501 unmutated common ancestor (UCA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2017-02-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.184, 71.821, 87.683 |
| Unit cell angles | 90.00, 110.09, 90.00 |
Refinement procedure
| Resolution | 35.920 - 2.020 |
| R-factor | 0.2219 |
| Rwork | 0.218 |
| R-free | 0.27190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iie |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rpim | 0.063 | 0.321 |
| Number of reflections | 29777 | 1161 |
| <I/σ(I)> | 15.4 | 1.7 |
| Completeness [%] | 99.8 | 75.5 |
| Redundancy | 7.8 | 2 |
| CC(1/2) | 0.968 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.095 M sodium citrate, pH 5.6, 20% isopropanol, 20% PEG4000 |
