6P38
Crystal Structure Analysis of TAF1 Bromodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.890, 95.890, 94.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.299 - 2.800 |
| R-factor | 0.2064 |
| Rwork | 0.206 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uv5 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.300 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.095 | 1.618 |
| Rpim | 0.039 | 0.606 |
| Total number of observations | 78453 | 4220 |
| Number of reflections | 12438 | 612 |
| <I/σ(I)> | 9.7 | 1.1 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 6.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 2000, Trimethylamine N-oxide dihydrate, Tris pH 8.5 |
