6P35

2.5 Angstrom structure of wild type Glyoxylate/Hydroxypyruvate reductase A from Escherichia Coli in complex with 2-keto arginine and NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 65 2 2
Unit cell lengths	158.578, 158.578, 98.285
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.780 - 2.495
R-factor	0.1733
Rwork	0.171
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kbo
RMSD bond length	0.011
RMSD bond angle	1.209
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.780	2.584
High resolution limit [Å]	2.495	2.495
Number of reflections	25774	2492
<I/σ(I)>	29.51
Completeness [%]	99.7
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	17% PEG 3350, 0.2 M Li2SO4, 0.1 M BIS-TRIS pH 5.5, 1.8 mM NADP