6P0Z
Crystal structure of N-acetylated KRAS (2-169) bound to GDP and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.194, 41.571, 115.410 |
| Unit cell angles | 90.00, 104.59, 90.00 |
Refinement procedure
| Resolution | 37.230 - 1.011 |
| R-factor | 0.1448 |
| Rwork | 0.144 |
| R-free | 0.16630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mbu |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.844 | 55.844 | 1.070 |
| High resolution limit [Å] | 1.010 | 3.030 | 1.010 |
| Rmerge | 0.056 | 0.043 | 0.693 |
| Rmeas | 0.061 | 0.047 | 0.798 |
| Total number of observations | 865327 | ||
| Number of reflections | 148845 | 5894 | 20076 |
| <I/σ(I)> | 14.84 | 38.98 | 1.45 |
| Completeness [%] | 95.3 | 97.9 | 79.8 |
| Redundancy | 5.814 | 6.433 | 3.514 |
| CC(1/2) | 0.998 | 0.998 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM BS1 pH 6.5, 100mM AminoAcids, 30% PEG500MME_P20K |
