6P0T

Crystal structure of ternary DNA complex "FX(1-2)-1Xis" containing E. coli Fis and phage lambda Xis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	108.190, 108.190, 152.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.255 - 3.603
R-factor	0.2017
Rwork	0.199
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p0u
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX ((1.15_3459))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.255	88.255	3.700
High resolution limit [Å]	3.600	16.110	3.600
Rmerge	0.118	0.055	0.816
Rmeas	0.123	0.058	0.850
Total number of observations	140007
Number of reflections	10977	161	753
<I/σ(I)>	13.14	29.94	3.09
Completeness [%]	99.8	100	97.2
Redundancy	12.755	10.193	12.697
CC(1/2)	0.999	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	15% PEG 4000