6P0S
Crystal structure of ternary DNA complex "FX2" containing E. coli Fis and phage lambda Xis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97170 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 107.670, 107.670, 150.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.550 - 2.700 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6p0t |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (1.3.2) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 87.550 | 87.550 | 2.770 |
High resolution limit [Å] | 2.700 | 12.090 | 2.700 |
Rmerge | 0.116 | 0.123 | 0.492 |
Rmeas | 0.121 | 0.130 | 0.534 |
Total number of observations | 222119 | ||
Number of reflections | 23763 | 343 | 1528 |
<I/σ(I)> | 12.53 | 19.34 | 3.73 |
Completeness [%] | 95.6 | 100 | 85.1 |
Redundancy | 9.347 | 11.534 | 6.57 |
CC(1/2) | 0.998 | 0.998 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 15% PEG 4000 |