6OZY

Wild type GapR crystal structure 2 from C. crescentus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 2 2 21
Unit cell lengths	39.770, 59.370, 93.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.970 - 2.014
R-factor	0.2352
Rwork	0.233
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.428
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.970	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.095	0.808
Number of reflections	10203	50
<I/σ(I)>	16.5	3.1
Completeness [%]	66.5	4.5
Redundancy	8.5	0.5
CC(1/2)	0.998	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	295	0.16 M ammonium sulfate 12 % PEG 3350 10 mM cadmium chloride