6OZU
Crystal structure of the MIF4G domain of Trypanosoma cruzi translation initiation factor EIF4G5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.051 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.100, 70.100, 303.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 2.400 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 30916 | 62721 |
| <I/σ(I)> | 19.66 | 0.94 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 12.7 | 13 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | sodium cacodylate 0.1 M, ammonium sulfate 2 M |
