6OZE

Crystal structure of the catalytic domain of human Endonuclease V (C140S/C225S/C226A/C228S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	50.949, 50.949, 102.978
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.729 - 1.500
R-factor	0.1729
Rwork	0.172
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w35
RMSD bond length	0.006
RMSD bond angle	0.823
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.21)
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.730	24.730	1.520
High resolution limit [Å]	1.500	8.210	1.500
Total number of observations	47096	131	2073
Number of reflections	47096	131	2073
<I/σ(I)>	9.9	21.4	2
Completeness [%]	98.2	44.9	88.3
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.6 M sodium acetate