6OZ2

Crystal structure of the broadly neutralizing antibody N49P6 Fab in complex with HIV-1 Clade A/E strain 93TH057 gp120 core.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	65.436, 80.815, 195.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.327 - 2.550
R-factor	0.2212
Rwork	0.218
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bck
RMSD bond length	0.011
RMSD bond angle	1.291
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.276	0.727
Rpim	0.166	0.556
Number of reflections	31853	1524
<I/σ(I)>	6.4	1.1
Completeness [%]	92.0	89.3
Redundancy	3.1	2.2
CC(1/2)	0.990	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	15% PEG 3350 0.1 M Tris-HCl pH 8.0