6OYC
Glycosylation Associate Protein (Gap123) complex from Streptococcus agalactiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 103.009, 168.321, 100.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.272 - 2.351 |
R-factor | 0.1822 |
Rwork | 0.181 |
R-free | 0.22670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6E2I |
RMSD bond length | 0.008 |
RMSD bond angle | 0.928 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_3051: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.272 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 72701 | 7084 |
<I/σ(I)> | 28.13 | |
Completeness [%] | 99.5 | |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES pH 7.5, 5% PEG 400, 2M Ammonium sulfate |