6OXI
Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (UAA)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 214.676, 453.509, 609.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 146.724 - 3.495 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v5c |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 146.724 | 146.724 | 3.590 |
| High resolution limit [Å] | 3.500 | 15.630 | 3.500 |
| Rmerge | 0.213 | 0.016 | 1.136 |
| Rmeas | 0.255 | 0.019 | 1.362 |
| Total number of observations | 2365932 | ||
| Number of reflections | 729135 | 8376 | 51384 |
| <I/σ(I)> | 5.98 | 43.76 | 1.07 |
| Completeness [%] | 98.0 | 93.9 | 93.9 |
| Redundancy | 3.245 | 3.237 | 3.251 |
| CC(1/2) | 0.994 | 1.000 | 0.352 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.1 M Tris-HAc, 0.2 M KSCN PEG 5.2%, 5.2% PEG550MME |
