6OXI

Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (UAA)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	214.676, 453.509, 609.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	146.724 - 3.495
R-factor	0.217
Rwork	0.216
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5c
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	146.724	146.724	3.590
High resolution limit [Å]	3.500	15.630	3.500
Rmerge	0.213	0.016	1.136
Rmeas	0.255	0.019	1.362
Total number of observations	2365932
Number of reflections	729135	8376	51384
<I/σ(I)>	5.98	43.76	1.07
Completeness [%]	98.0	93.9	93.9
Redundancy	3.245	3.237	3.251
CC(1/2)	0.994	1.000	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.1 M Tris-HAc, 0.2 M KSCN PEG 5.2%, 5.2% PEG550MME