6OX4

A SETD3 Mutant (N255A) in Complex with an Actin Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	60.393, 177.039, 65.913
Unit cell angles	90.00, 92.50, 90.00

Refinement procedure

Resolution	36.733 - 2.294
R-factor	0.1896
Rwork	0.188
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mbj
RMSD bond length	0.003
RMSD bond angle	0.530
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.733	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.247	0.682
Rpim	0.112	0.437
Number of reflections	58449	4402
<I/σ(I)>	8.8	1.6
Completeness [%]	95.2	75
Redundancy	5
CC(1/2)		0.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	292	0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000