6OWJ

Zn-mediated polymerization of human SFPQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	61.230, 62.450, 67.880
Unit cell angles	90.00, 95.74, 90.00

Refinement procedure

Resolution	27.380 - 1.940
R-factor	0.178
Rwork	0.176
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wii
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.380	1.990
High resolution limit [Å]	1.940	1.940
Rmerge	0.062	0.600
Rpim	0.042	0.401
Number of reflections	37507	2239
<I/σ(I)>	10.3	1.6
Completeness [%]	99.2
Redundancy	4.1
CC(1/2)	0.998	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14% PEG4000, 0.2 M calcium chloride, 0.1 M MES, pH 6.0