6OWD
Arginine Containing Reengineered Coiled-Coiled Dimer to Examine the Impact of Proximal Cation Identity on Hydrophobically-Driven Assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.1272 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.730, 31.290, 23.930 |
| Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
| Resolution | 36.590 - 1.500 |
| Rwork | 0.184 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dzm |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.590 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.109 | 1.003 |
| Rmeas | 0.118 | 1.086 |
| Rpim | 0.046 | 0.411 |
| Number of reflections | 8381 | 808 |
| <I/σ(I)> | 12.09 | 1.34 |
| Completeness [%] | 95.0 | 93.73 |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.997 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | No salt, 0.1 M Bis-Tris pH 5.5, 3.0 M sodium chloride |
