6OW7

X-ray Structure of Polypeptide Deformylase with a Piperazic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	173
Detector technology	CCD
Collection date	2005-12-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 43
Unit cell lengths	69.229, 69.229, 91.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.280 - 1.450
R-factor	0.2063
Rwork	0.206
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	HKL-2000
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.280	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	72134	5088
<I/σ(I)>	42.6
Completeness [%]	94.0	66.6
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	1 % - 4 % PEG400 45 % - 55% Ammonium Sulfate 0.1 M HEPES