6OW3
X-ray crystal structure of a bacterial reiterative transcription complex of pyrG promoter variant -1T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.187, 101.833, 295.755 |
| Unit cell angles | 90.00, 98.64, 90.00 |
Refinement procedure
| Resolution | 36.871 - 2.766 |
| R-factor | 0.2177 |
| Rwork | 0.217 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vo8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rpim | 0.044 | 0.514 |
| Number of reflections | 136486 | 5466 |
| <I/σ(I)> | 16.24 | 1.29 |
| Completeness [%] | 97.7 | 78.2 |
| Redundancy | 3.8 | 3.1 |
| CC(1/2) | 0.997 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 9.5 % PEG4000 |
