6OVV
Coiled-coil Trimer with Glu:4-pyridinylalanine:Lys Triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-04 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | H 3 |
| Unit cell lengths | 38.379, 38.379, 119.428 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.290 - 2.201 |
| R-factor | 0.194 |
| Rwork | 0.186 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2906) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.290 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.201 |
| Rpim | 0.083 | 0.342 |
| Number of reflections | 3330 | 342 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.993 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 40% PEG300, 100 mM sodium phosphate dibasic/citric acid, pH 4.2 |
