6OVO

Crystal structure of the unliganded PG10 TCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 61
Unit cell lengths	149.620, 149.620, 55.991
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.826 - 2.490
R-factor	0.179
Rwork	0.177
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wjo
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.810	2.620
High resolution limit [Å]	2.490	2.490
Rmerge	0.148
Rpim	0.048	0.402
Number of reflections	25252	2502
<I/σ(I)>	11.2	2.2
Completeness [%]	99.6	99.6
Redundancy	10.2	7.6
CC(1/2)	0.996	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Ammonium Sulfate