6OVN

Crystal structure of the unliganded Clone 2 TCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 43
Unit cell lengths	75.188, 75.188, 109.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.820 - 1.770
R-factor	0.1714
Rwork	0.170
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g8f
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.820	1.810
High resolution limit [Å]	1.770	1.770
Rmerge	0.076
Rmeas	0.084
Rpim	0.037	0.396
Number of reflections	59111	3375
<I/σ(I)>	18	1.9
Completeness [%]	100.0	100
Redundancy	10.4	10.5
CC(1/2)	0.999	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350