6OVD

Crystal structure of GluN1/GluN2A NMDA receptor agonist binding domains with glycine and antagonist, 3-ethylphenyl-ACEPC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	.979
Spacegroup name	P 21 21 21
Unit cell lengths	54.666, 87.237, 122.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.850 - 2.102
R-factor	0.1967
Rwork	0.194
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i57
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.850	2.177
High resolution limit [Å]	2.100	2.100
Rmerge	0.119	0.517
Rmeas	0.133	0.602
Rpim	0.058	0.300
Number of reflections	34185	3090
<I/σ(I)>	8.59	2.31
Completeness [%]	98.0	90.59
Redundancy	4.6	3.5
CC(1/2)	0.993	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M AMMONIUM SULFATE AND 16-22% PEG 4000