6OV1

Structure of Staphylococcus aureus RNase P protein mutant with defective mRNA degradation activity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-07-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0322
Spacegroup name	P 1
Unit cell lengths	40.386, 43.467, 45.837
Unit cell angles	91.11, 114.83, 110.95

Refinement procedure

Resolution	40.761 - 1.660
R-factor	0.1905
Rwork	0.187
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d6t
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.720
High resolution limit [Å]	1.657	3.580	1.660
Rmerge	0.042	0.027	0.513
Rmeas	0.059	0.038	0.725
Rpim	0.042	0.027	0.513
Number of reflections	29214	2983	2838
<I/σ(I)>	6.8
Completeness [%]	95.2	97.1	92.2
Redundancy	1.8	1.8	1.8
CC(1/2)		0.996	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298.15	0.1 M Imidazole-HCl, pH8 1.3 M Sodium Citrate