6OUY

The crystal structure of the isolate tryptophan synthase alpha-chain from Salmonella enterica serovar typhimurium at 1.60 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-11-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	70.910, 70.910, 203.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.595 - 1.600
R-factor	0.179
Rwork	0.177
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6oso
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP (11.7.01)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.410	19.595	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge	0.070	0.022	0.667
Rmeas	0.078	0.025	0.756
Rpim	0.034	0.012	0.348
Total number of observations		6224	25936
Number of reflections	40745	1418	5741
<I/σ(I)>	9.8	26.7	1.5
Completeness [%]	99.6	96.6	98.1
Redundancy	5	4.4	4.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	1.5-2.0 M ammonium sulfate