6OUS

Structure of fusion glycoprotein from human respiratory syncytial virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-17
Detector	RAYONIX MX300HE
Wavelength(s)	0.979490
Spacegroup name	P 1
Unit cell lengths	118.153, 126.595, 148.165
Unit cell angles	87.24, 79.15, 86.29

Refinement procedure

Resolution	49.860 - 3.400
Rwork	0.245
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mmt 1hzh
Data reduction software	XDS
Data scaling software	Aimless (0.3.6)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.860	49.860	4.160
High resolution limit [Å]	3.400	5.890	3.400
Rmerge	0.127	0.044	0.344
Rmeas	0.179	0.062	0.486
Rpim	0.127	0.044	0.344
Total number of observations	228565	44035	104047
Number of reflections	114498	22078	52095
<I/σ(I)>	3.9	6.6	2.2
Completeness [%]	99.0	99.2	98.8
Redundancy	2	2	2
CC(1/2)	0.980	0.994	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris 8.5, 10% PEG 8000, 200 mM ammonium sulfate