6OU1
Crystal Structure of the Computationally-derived 21-Variant of the Myocilin Olfactomedin Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-03-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.357, 66.889, 80.999 |
Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
Resolution | 36.712 - 1.878 |
R-factor | 0.1692 |
Rwork | 0.168 |
R-free | 0.19510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.637 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.712 | 1.950 |
High resolution limit [Å] | 1.878 | 1.878 |
Rmerge | 0.102 | |
Number of reflections | 34931 | 2963 |
<I/σ(I)> | 11.98 | 2.49 |
Completeness [%] | 91.0 | |
Redundancy | 4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 3350, 0.2 M Bis Tris pH 6.0, 0.1 M Magnesium formate |