6OTZ
Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and (+)FTC-TP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 167.069, 170.368, 103.188 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.821 - 2.857 |
R-factor | 0.2002 |
Rwork | 0.198 |
R-free | 0.24070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6or7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.625 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.030 |
High resolution limit [Å] | 2.857 | 2.857 |
Rpim | 0.075 | 0.430 |
Number of reflections | 34440 | 5442 |
<I/σ(I)> | 12.54 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 12.8 | |
CC(1/2) | 0.996 | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0 |