6OTR

Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (AAU)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	213.364, 451.705, 607.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	146.148 - 3.120
R-factor	0.2327
Rwork	0.232
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v5c
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	146.148	3.200
High resolution limit [Å]	3.120	3.120
Rmerge	0.209	0.827
Rmeas	0.245	1.580
Total number of observations	479790
Number of reflections	1014922	282553
<I/σ(I)>	6.59	1.12
Completeness [%]	98.6	86.4
Redundancy	3.671	2.422
CC(1/2)	0.994	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.1M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME