6OSN

Potent and Selective Antitumor Antibody Targeting a Membrane-Proximal Epitope of ROR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	33.606, 41.917, 52.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.141 - 1.083
R-factor	0.1533
Rwork	0.153
R-free	0.16090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ba5
RMSD bond length	0.008
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.671	1.122
High resolution limit [Å]	1.021	1.083
Rmerge	0.021	0.059
Rmeas	0.025	0.079
Rpim	0.013	0.051
Number of reflections	29243	29243
<I/σ(I)>	35.5
Completeness [%]	76.7
Redundancy	3.1
CC(1/2)	0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M Li acetate 20% PEG 3350