6OSH

Potent and Selective Antitumor Antibody Targeting a Membrane-Proximal Epitope of ROR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978720
Spacegroup name	P 21 21 21
Unit cell lengths	45.165, 67.201, 89.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.332 - 1.117
R-factor	0.1622
Rwork	0.162
R-free	0.16920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ba5
RMSD bond length	0.008
RMSD bond angle	0.983
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.330	1.157
High resolution limit [Å]	1.117	1.117
Rmerge	0.045	0.561
Rmeas	0.050	0.756
Rpim	0.022	0.502
Number of reflections	81433	4074
<I/σ(I)>	17.3
Completeness [%]	89.0
Redundancy	4.9
CC(1/2)	0.999	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M Na citrate tribasic 20% PEG3350