6ORT

Crystal Structure of Bos taurus Mxra8 Ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-16
Detector	NOIR-1
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	77.530, 77.530, 242.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.981 - 2.300
R-factor	0.2193
Rwork	0.218
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nk3
Data reduction software	XDS (20180126)
Data scaling software	Aimless (0.5.26)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.470	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.144	2.234
Rmeas	0.146	2.248
Number of reflections	20081	1939
<I/σ(I)>	49.2	3.7
Completeness [%]	99.9	99.95
Redundancy	71.1	76.6
CC(1/2)	1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M HEPES, pH 8.0, 6% w/v PEG6000, 1.0 M lithium chloride