6ORK

Crystal structure of Sel1 repeat protein from Oxalobacter formigenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-23
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	61.926, 103.368, 122.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.585 - 3.000
R-factor	0.2114
Rwork	0.209
R-free	0.25380
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.087	0.034	0.893
Rmeas	0.096	0.037	1.012
Rpim	0.039	0.016	0.461
Total number of observations	93909
Number of reflections	16196	922	663
<I/σ(I)>	6.7
Completeness [%]	98.1	99.5	83.2
Redundancy	5.8	5.7	3.8
CC(1/2)		0.998	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	10 mM tri-sodium citrate, 33% PEG6000